Part:BBa_K2959000

Expressible LL 37 - Cathelicidin

This composite part enables tha expression of the human antimicrobial peptide LL 37 in E. coli BL21 (DE3). The BioBrick consists of a T7 Promoter + RBS + LL 37 sequence + double terminator (rrnBT1 + T7TE). The T7 promoter, inducible through IPTG, controls the expression of the part.

Usage and Biology

The protein LL-37 cathelicidin is a human antimicrobial peptide active against Gram-positive bacteria, Gram-negative bacteria and fungi¹. LL-37 is the only known cathelicidin peptide produced in humans; its structure is amphipathic and has an α-helical conformation with a positive charge. Talking about its mechanism of action, the cathelicidins targeted the cell membrane and causes its complete disruption inducing cell membrane permeabilization and simultaneous vacuolar expansion. Intracellular activities of the peptide may contribute to its antifungal activity².

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

1. Tollin, M. & Bergman, P. (2003). Antimicrobial peptides in the first line defence of human colon mucosa. (4):523-30 DOI:10.1016/s0196-9781(03)00114-1

2. Ordonez, S. R., Amarullah, I. H., Wubbolts, R. W., Veldhuizen, E. J., & Haagsman, H. P. (2014). Fungicidal mechanisms of cathelicidins LL-37 and CATH-2 revealed by live-cell imaging. Antimicrobial agents and chemotherapy, 58(4), 2240-2248. DOI: 10.1128/AAC.01670-13